Influence of hepatotropic and metabiotic correction on the spectrum of free fatty acids in experimental toxic liver damage

Authors

DOI:

https://doi.org/10.22141/2308-2097.56.2.2022.492

Keywords:

carbon tetrachloride, hepatobioptates, monounsaturated free fatty acids, rats

Abstract

Background. Today most of the scientists are studying the processes of hepatocyte damage under the influence of free fatty acids (FFA) in vitro conditions. Therefore, in vivo studies of the spectrum of FFA in liver pathologies of different genesis, including toxic ones, are of considerable interest. Materials and methods. Toxic liver damage was simulated by subcutaneous injection of CCl4 solution in olive oil into rats. The following groups were formed: control (healthy; n = 15); I — CCl4-induced liver damage without drug correction (n = 6); III — CCl4-induced liver damage + metadoxin (Liveria IC; n = 8); III — CCl4-induced liver damage + metabiotic (Hilac forte) (n = 7). For morphological assessment of steatosis and fibrosis we used three-color qualitative staining of liver samples by Mallory-Slinchenko. Quantitative content of monounsaturated fatty acids (MUFA) in liver homoge­nate was determined by gas chromatography. Results. Evaluation of hepatobioptates in group I rats revealed tissue disorganization with macrovesicular steatosis in the cytoplasm of hepatocytes, the formation of interparticle multiple fibrous septa and inflammatory cell infiltration. The use of metadoxin (group II) and metabiotic (group III) improved the morphological picture of the liver, which was damaged by CCl4. Total MUFA content increased significantly in 118 (p < 0.001), 34 (p < 0.001) and 99 times (p < 0.001), respectively, for groups I–III animals relative to control, but in group II — tended to decrease in 3.5 (p = 0.430) and 2.9 times (p = 0.064), compared to groups I and III, respectively. Conclusions. It was found that correction with methadoxin and metabioticreduced the manifestations of protein-fatty dystrophy in hepatocytes. In all animal research groups, the content of PUFA increased mainly due to significant concentrations of cis-10-pentadecenoic, cis-10-heptadecenoic, trans- and cis-9-octadecenoic and cis-11-eicosenoic acids. MUFA content decreased more under the influence of methadoxin than metabiotic.

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Published

2022-06-16

How to Cite

Didenko, V., Karachynova, V., Klenina, I., Gaidar, Y., Oshmianska, N., Hrabovska, O., Halinskyi, O., & Vishnarevskaya, N. (2022). Influence of hepatotropic and metabiotic correction on the spectrum of free fatty acids in experimental toxic liver damage. GASTROENTEROLOGY, 56(2), 82–88. https://doi.org/10.22141/2308-2097.56.2.2022.492

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