Research of Melatonin Level in Rats of Different Sexes and Age with Ulcerative Lesion of the Stomach
The aim of the study was to determine of melatonin level in the blood serum in rats of different sexes and age in ulcerative lesions of the stomach. Materials and methods. Using the method of alcohol-prednisolone injury, we have simulated ulcerative lesions of the stomach. The level of melatonin in the blood serum in rats of different age and sexes with ulcerative lesions of the stomach was determined by enzyme-linked immunosorbent assay. Experimental animals were divided into groups by the age, namely 3, 9, 15 and 20 months, corresponding to the human age of 14, 29–30, 43–44, 55–56 years, respectively. Results. In the course of the research, it was found that rats with the ulcerative lesions of the stomach had significant (r ≤ 0.05) reduction of serum melatonin in animals of all experimental groups. In males, the lowest levels of melatonin in ulcerative lesion were in groups of 9 and 20 months — (174.0 ± 12.9) pmol/L and (141.5 ± 21.4) pmol/L, respectively. The level of melatonin in males aged 9 months was significantly lower (p ≤ 0.05), by 26 %, than in rats aged 3 months, and by 16 % — compared with rats aged 15 months (p ≤ 0.05). In rats aged 20 months, melatonin levels were reduced by 40 and 32 % with respect to experimental groups of animals aged 3 and 15 months (p ≤ 0.05). Relatively to the control group, among male rats the greatest reduction also observed in animals ages 20 months — 43 % and 9 months — 39 % (p ≤ 0.05). In male rats aged 3 months, melatonin level was lower by 22 %, and in the age group of 15 months — by 29 % relative to control animals (p ≤ 0.05). In females, reduction of melatonin levels occurred gradually with age: from the highest in females aged 3 months to significantly lowest compared to other age groups — females aged 20 months in the control and experimental groups. The females rats with ulcerative gastric lesions had a decrease in melatonin levels by 21–23 % relative to controls in all age groups (p ≤ 0.05). Discussion. The results indicate that ulcerative lesions of the stomach is likely to result in injury of enterochromaffin cells of the gastric mucosa, accompanied by a significant reduction in levels of melatonin in rats of both sexes and all age groups. Thus, it was found that despite no significant difference between levels of melatonin in males and females on the background of ulcerative lesions of the stomach, the overall level of melatonin reduction in males is 22–43 % higher relative to controls than in females — 21–23 %. The biggest gender differences in melatonin levels decrease were found at the age of 9 months (corresponds to the human age of 29–30 years) — by 39 % in males and by 23 % in females relative to controls and 20 months (corresponds to the human age of 55–56 years) — 43 and 22 %, respectively. The results suggest that in men aged 29–30 and 55–56 years, ulcerative lesions result in significant damage to extrapineal sources of melatonin synthesis and more severe course of the stomach ulcer with the development of complications. Conclusions. 1. Ulcerative lesions of the stomach lead to reduce levels of melatonin in the blood serum in rats of different sexes and age. 2. The greatest reduction in levels of melatonin in experimental gastric ulcer occurs in rat males aged 9 and 20 months corresponding to the human age of 29–30 and 55–56 years.
Full Text:PDF (Українська)
Sirby I.F. Differencialnaia diagnostica gastoduodenalnoy yazvy I ostrogo pankreatita [Differential diagnosis of gastroduodenal ulcers and acute pancreatitis]. Zaporojskii medichnii jurnal [Zaporozhye medical journal], 2012, vol. 1, no 70, pp. 35–36 (in Russian).
Islamova E.A. Vozrastnie osobenosti yazvenoi bolezni zheludka I dvenadcatiperstnoi kishki [Age features of peptic and duodenal ulcer disease]. Saratovskii naychno-medicinskii jurnal [Saratov Journal of Medical Scientifi Research], 2009, vol. 5, no 4, pp. 569–571 (in Russian).
Lazernic L.B., Drozdov V.N. Klinicheskie proiavlenia bolezni I pozhiloi vozrast [The clinical manifestations of disease and old age]. Moscow, Anarsis, 2003, pp. 25–34 (in Russian).
Wu H.C., Tuo B.G., Wu W.M., Gao Y., Xu Q.Q., Zhao K. Prevalence of peptic ulcer in dyspeptic patients and the influence of age, sex, and Helicobacter pylori infection. Digestive diseases and sciences, 2008, Vol. 53, Issue 10, pp. 2650 – 2656.
Ivashkin V.T., Komarov F.I., Rappoport S.I. Kratkoe rukovodstvo po gastroenterologii [Quick Reference Guide on gastroenterology]. Moscow, 2001, 457 p. (in Russian).
Tsimmerman Ya. S. Yazvenaiia bolezn: aktualmie problemi etiologii, patogeneza, differencialnogo lechenia [Ulcer disease: challenging problems of etiology, pathogenesis, differential treatment]. Klinichna medicina [Clinical medcine], 2012, no 8, pp. 11–18 (in Russian).
Xin Z., Jiang S., Jiang P. et al. Melatonin as a treatment for gastrointestinal cancer: a review. J. Pineal Res., 2015, Vol. 58, Issue 4, pp. 375–387.
Jaworek J., Leja-Szpak A. Melatonin influences pancreatic cancerogenesis. Histol Histopathol, 2013, Vol. 29, Issue 4, pp. 423–431.
Laothong U., Pinlaor P., Boonsiri P. et. al. Melatonin inhibits cholangiocarcinoma and reduces liver injury in Opisthorchis viverrini-infected and N-nitrosodimethylamine-treated hamsters. J. Pineal. Res., 2013, Vol. 55, Issue 3, pp. 257–266.
Konturek S.J., Konturek P.C., Brzozowski T. Melatonin in gastroprotection against stress-induced acute gastric lesions and in healing of chronic gastric ulcers. Journal of physiology and pharmacology, 2006, Vol. 57, Issue 5, pp. 51 – 66.
Arendt J. Melatonin and human rhythms. Chronobiology International, 2006, Vol. 23, Issue 1&2, pp. 21–37.
Waterhouse J., Reilly T., Atkinson G., Edwards B. Jet lag: trends and coping strategie. The Lancet, 2007, Vol. 369, Issue 9567, pp. 1117 – 1129.
Gubin D.G. Okolonedelnie (cirkaseptalnie) ritmi v fiziologii (obzor) [About-weekly (circaseptan) rhythms in physiology (review)]. Yspehi sovremenogo estestvoznaniia [Advances in current natural sciences], 2015, Isuue 1, part 8, pp. 1268 – 1272 (in Russian).
Kvetnoi I.I., Raikhlin N.T., Iuzhakov V.V., Ingel I.E. Enterochromaffinie kletki – osnovnoy istochnic melatonina v organizme [Enterochromaffin cells - the main source of melatonin in the body]. Bulleten eksperimentalnoi Biologii I medicini [Bulletin of Experimental Biology and Medicine], 1999, Vol. 127, Issue 4, pp. 366–370 (in Russian).
Knyazkin I.V. Ekstrpinealnii melatonin v protsesakh uskorennogo I prezhdevremenogo starenia u kris [Extrapineal melatonin in processes of accelerated and premature aging in rats]. Uspekhi gerantologii [Adv. Gerontol.], 2008, Vol. 21, no 1, pp. 80–82 (in Russian).
Zvyagintseva T.D., Gamanenko Y.K. Morfofenktcionalnie osobenosti tuchnikh kletok slizistoy obolochki jeludka pri khronocheskikh eroziyach [Morphological-functional features corpulence cells of mucous membrane of stomach in chronics erosions]. Krimskii terapevticheskii jurnal [Crimean Journal of Internal Diseases], 2008, Vol. 1, Issue 1, pp. 82–84 (in Russian).
Stefanov O.V. Doklinichni doslidjennya likarskikh zasobiv. Metod. Rekomendatcii [Preclinical studies of medicines. Method. recommendations]. Kiev, Avitcena, 2001, pp.323 (in Ukraine).
Kochetov A.G., Lyang O.V., Masenko V.P., et al. Metodi statisticheskoi obrabotki meditcinskikh danikh [Methods of statistical processing of medical data]. Moscow, RKNPK, 2012. 42 p. (in Russian).
Oh Sumin, Oh Sejong Epidemiologacal and genome-wide association study of gastritis or gastric ulcer in Korean populations. Genomics & Informatics, 2014, Vol. 12, Issue 3. pp. 127–133.
Thorsen K., Soreide J. A., Kvalov J. T., et al Epidemiology of perforated peptic ulcer: age- and gender-adjusted analysis of incidence and mortality. World J. Gastroenterol, 2013, Vol. 19, Issue 3, pp. 347–354.
- There are currently no refbacks.
Copyright (c) 2016 GASTROENTEROLOGY
This work is licensed under a Creative Commons Attribution 4.0 International License.
© Publishing House Zaslavsky, 1997-2018