The Role of Enteropathogenic E.coli in the Development and Progression of Chronic Non-Ulcerative Colitis
Objective. The analysis of the literature has shown that in patients with chronic non-ulcerative colitis, there were isolated E.coli that cause dysentery-like, cholera-like diseases and escherichioses. Thus, the aim of our study was to establish the level of persistence of enteropathogenic Escherichia in the colon of patients with chronic non-ulcerative colitis and their importance in the ecological system «macroorganism — microbiota». Material and methods. During 2000–2015, there has been conducted a bacteriological examination of the colon content in patients with chronic non-ulcerative colitis aged 27–41 years (average age 37.74 ± 3.62 years). The resulting cultures were examined in the indicative agglutination tests with OKA polyvalent serum. Positive cultures were tested with polyvalent serum of narrow spectrum ОКВ, ОКС, ОКD and ОКЕ. Results. In patients, in colon cavity of which E.coli O18ac:K77; O26:K60; O55:K59; O128ab:K67 were detected, the disease occurs by nosology of colienteritis. In those patients, in whom O25:K11; O144:K; O124:K72 serotypes have been identified, disease occurred as bacterial dysentery, and O25:K11 and O128:K67 — in cholera-like form. Discussion. In 94.50 % of patients, common E.coli were detected, but in 55 (35.03 %) of them enteropathogenic Escherichia persist, in 41 (26.11 %) — E.coli Hly+, in 37 (23.57 %) — E.coli Lac–, in 18 (11.46 %) — enterotoxigenic E.coli, in 14 (8.42 %) — enteroinvasive, and in 11 (7.01 %) — enterohemorragic Escherichia. Conclusions. It was determined that the main serotypes of opportunistic Escherichia, which colonize and persist in the colon cavity of patients with chronic non-ulcerative colitis, were O114:K90; O25:K11; O124:K72; O128:K67; O18ac:K77, the persistence of which affects clinical manifestation from colitis to dysentery-like or cholera-like disease.
Belousova O.Yu. Khronichnyy nespetsyfichnyy nevyrazkovyy kolit u ditey (etiolohiya, patohenez, klasyfikatsiya, klinichni proyavy, diahnostyka, likuvannya, dyspanseryzatsiya) Chronic non-specific non-ulcer colitis in children (etiology, pathogenesis, classification, clinical manifestations, diagnosis, treatment, clinical examination): [Metod. rekomend]. Khark. med. akad. pislyadypl. osvity MOZ Ukr. K., 2007. 19 р.
Holovatyuk L.M. Rol' etiolohichnykh chynnykiv u rozvytku ushkodzhen' tovstoyi kyshky [The role of the etiological factors in the development of colon injuries]. Visnyk morfolohiyi, 2015, no.2, рр. 537-541.
Lopach S.Y., Chubenko A.V., Babych N.N., Statystycheskye metodы v medyko-byolohycheskykh yssledovanyyakh s yspol'zovanyem Exel [Statistical methods in biomedical research using Excel]. K., MORYON, 2000. 320 p.
Unified protocol of care for children with chronic ulcerative colitis. Unifikovanyy protocol of care for children with chronic ulcerative colitis [MOH Ukraine № 59 "On approval of standardized clinical protocols of medical care for children with diseases of the digestive system"] 01.29.2013.
Cai K., Tu W., Liu Y. [Novel fusion antigen displayed-bacterial ghosts vaccine candidate against infection of Escherichia coli O157:H7]. Sci Rep., 2015, vol. 5, no. 2, pp. 174-179. doi: 10.1038/srep17479. Available at: http://www.ncbi.nlm.nih.gov/pubmed/26626573
Dormanesh B., Siroosbakhat S., Karimi Goudarzi P. [Shiga Toxigenic Escherichia coli in Iranian Pediatric Patients With and Without Diarrhea: O-Serogroups, Virulence Factors and Antimicrobial Resistance Properties]. Iran Red Crescent Med J., 2015, vol.10, no. 17, pp. 297-306. doi: 10.5812/ircmj.29706. eCollection 2015. Available at: http://www.ncbi.nlm.nih.gov/pubmed/26566453
Desilets M., Deng X., Rao C., Desilets M. [Genome-based Definition of an Inflammatory Bowel Disease-associated Adherent-Invasive Escherichia coli Pathovar]. Inflamm Bowel Dis., 2016, vol. 1, no. 22, pp. 1-12. doi: 10.1097/MIB.0000000000000574. Available at: http://www.ncbi.nlm.nih.gov/pubmed/26444104
Strus M., Okoń K., Nowak B. [Distinct effects of Lactobacillus plantarum KL30B and Escherichia coli 3A1 on the induction and development of acute and chronic inflammation]. Cent Eur J Immunol., 2016, vol. 4, no. 40, pp. 420-430. doi: 10.5114/ceji.2015.56963. Epub 2016 Jan 15. Available at: http://www.ncbi.nlm.nih.gov/pubmed/2686230
Copyright (c) 2016 GASTROENTEROLOGY
This work is licensed under a Creative Commons Attribution 4.0 International License.
© Publishing House Zaslavsky, 1997-2018