Population changes of colon lumen microbiota in patients with chronic hepatitis C

D.V. Rotar, L.I. Sydorchuk, A.S. Sydorchuk, S.E. Dejneka, I.I. Sydorchuk

Abstract


Background. Hepatitis C is one of the major problems of nosocomial infections associated with blood transfusions, administration of blood products, and medical manipulation with invasive diagnostic and therapeutic purposes. The purpose of our study was to establish population level of taxons of colon microbiota in patients with chronic hepatitis C. Materials and methods. 72 samples of colon content of patients with chronic hepatitis C, including 44 women, 28 men aged 21 to 53 years (average age (37.5 ± 1.7) years old) underwent microbiological investigation. Results. The study showed that the population of Bifidobacterium spp. in the colon lumen of patients with chronic hepatitis C decreased by 41.28 %, Lactobacillus spp. by 41.15 %, the coefficient of quantitative domination by 2.07 and 2.22 times, the significance coefficient by 3.25 and 3.36 times, respectively, that contributes to the population level increasing, coefficients of quantitative domination and significance of Bacteroides spp. by 65.01 % and 24.94 %, respectively, that means increased population level bacteria species of Escherichia and Enterococcus by 52.21 and 26.48 %, respectively, Peptostreptococcus spp. by 57.43 %, Clostridium spp. by 2.03 times. Conclusion. In colon lumen of patients with chronic hepatitis C there is formed distinct deficiency of autochthonous obligate Bifidobacterium spp. by 41.28 %, Lactobacillus spp. by 42.15 %, increased concentration of Bacteroides spp. in biotope by 65.01 %, Peptostreptococcus spp. by 57.43 %, Clostridium spp. by 2.03 times, and Escherichia bacteria by 52.21 % and Enterococcus by 26.48 %. Dysbiosis/dysbacteriosis of II–III stages (80.56 %) was diagnosed by the changes of population level of main, additional and accidental colon luminal microbiota in majority patients with chronic hepatitis C.

Keywords


hepatitis C; colon; microbiota; population level

References


Sandler NG, Koh C, Roque A, et al. Host response to translocated microbial products predicts outcomes of patients with HBV or HCV infection. Gastroenterology. 2011:141(4):1220-30.e3. doi: 10.1053/j.gastro.2011.06.063. 

Tabibian JH, Varghese C, LaRusso NF, O'Hara SP. The enteric microbiome in hepatobiliary health and disease. Liver int. 2016;36(4):480-7. doi: 10.1111/liv.13009. 

Aly AM, Adel A, El-Gendy AO, Essam TM, Aziz RK. Cut microbiome alterations in patients with stage 4 hepatitis C. Gut Pathog. 2016;8(1):42. doi: 10.1186/s13099-016-0124-2.

Minemura M, Shimizu Y. Gut microbiota and liver diseases. World J Gastroenterol. 2015;21(6):1691-1702. doi: 10.3748/wjg.v21.i6.1691

Schnabl B, Brenner DA. Interactions Between the Intestinal Microbiome and Liver Diseases. Gastroenterology. 2014;146(6):1513-24. doi: 10.1053/j.gastro.2014.01.020. 

 Benten D, Wiest R. Gut microbiome and intestinal barrier failure - the “Achilles heel” in hepatology? J. Hepatol. 2012;56:1221-1223. doi: 10.1016/j.jhep.2012.03.003. 

Schnabl B. Linking intestinal homeostasis and liver disease. Curr opin gastroenterol. 2014;29(3):264-70. doi: 10.1097/mog.0b013e32835ff948. 

Roderburg C, Luedde T. The role of the gut microbiome in the development and progression of liver cirrhosis and hepatocellular carcinoma. Gut Microbes. 2014;5 (5):441-5. doi: 10.4161/gmic.29599. 

Henao-Mejia J, Elinav E, Thaiss CA, Licona-Limon P, Flavell RA. Role of the intestinal microbiome in liver disease. J. Autoimmun. 2013;46:66-73. doi: 10.1016/j.jaut.2013.07.001. 

Son G, Kremer M, Hines IN. Contribution of Gut Bacteria to Liver Pathobiology. Gastroenterol Res Pract. 2010;2010. pii: 453563. doi: 10.1155/2010/453563. 

Lozupone CA, Stombaugh JI, Gordon JI, Jansson JK, Knight R. Diversity, stability and resilience of the human gut microbiota. Nature. 2012;489(7415):220-30. doi: 10.1038/nature11550.

 Xie G, Wang X, Liu P, et al. Distinctly altered gut microbiota in the progression of liver disease. Oncotarget. 2016 Apr 12;7(15):19355-66. doi: 10.18632/oncotarget.8466.

Fukui H. Gut Microbiota and Host Reaction in Liver Diseases. Microorganisms. 2015 Oct 28;3(4):759-91. doi: 10.3390/microorganisms3040759.

Brenner DJ, Krieg NR, Staley JT, editors. Bergey’s Manual of Systematic Bacteriology, Volume Two: The Proteobacteria Part C. New York: Springer-Verlag; 2005. 1388 p. doi: 10.1007/0-387-29298-5. 

Márquez M, Fernández Gutiérrez del Álamo C, Girón-González JA. Gut epithelial barrier dysfunction in human immunodeficiency virus-hepatitis C virus coinfected patients: Influence on innate and acquired immunity. World J Gastroenterol. 2016 Jan 28;22(4):1433-48. doi: 10.3748/wjg.v22.i4.1433.

Hetta HF, Mehta MJ, Shata MTM. Gut immune response in the presence of hepatitis C virus infection. World J Immunol. 2014;4(2):52-62. doi: 10.5411/wji.v4.i2.52. 

Schnabl B, Brenner DA. Interactions Between the Intestinal Microbiome and Liver Diseases. Gastroenterology. 2014 May;146(6):1513-24. doi: 10.1053/j.gastro.2014.01.020.

 Fouts DE, Torralba M, Nelson KE, Brenner DA, Schnabl B. Bacterial translocation and changes in the intestinal microbiome in mouse models of liver disease. J Hepatol. 2012 Jun;56(6):1283-92. doi: 10.1016/j.jhep.2012.01.019.

Munteanu D, Negru A, Radulescu M, Mihailescu R, Arama SS, Arama V. Evaluation of bacterial translocation in patients with chronic HCV infection. Rom J Intern Med. 2014;52(2):91-6. PMID: 25338345.

Baranovsky AYu, Kondrashyna EA. Dysbakteryoz y dysbyoz kyshechnyka [Intestinal dysbacteriosis and dysbiosis]. SPB: Pyter; 2000. 209p.




DOI: https://doi.org/10.22141/2308-2097.51.2.2017.101702

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 GASTROENTEROLOGY

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

© Publishing House Zaslavsky, 1997-2018

 

   Seo анализ сайта