State of IgG4-positive plasma cells in the colon mucosa of chronic inflammatory bowel disease

Yu.А. Gaidar, G.Yu. Arzhanova, A.P. Galenko


Background. The diagnosis of IgG4-associated sclerosing disease, IgG4-associatied condition, is based on a comprehensive evaluation of characteristic clinical, radiographic, serologic, histological and immunohistochemical features. The histopathological is the main examination in the diagnosis of IgG4-associatied diseases. The purpose of the study was to evaluate the state of IgG4-positive plasma cells in the mucosa of the colon in patients with established morphological and endoscopic diagnosis of ulcerative colitis (UC) and Crohn’s disease (CD). Materials and methods. The study used biopsies material from 14 patients treated at the Institute of Gastroenterology, in the department intestine diseases, with established morphological and endoscope diagnosis of UC (8) and CD (6) in the acute stage. All patients had no evidence of autoimmune pancreatitis type I and II. Biopsy were fixed in 10.0% neutral formalin, dehydrated in alcohols of increasing concentration and embedded in paraffin for histological studies. Histological sections of 3–5 µm were colored with hematoxylin and eosin. There were used monoclonal IgG4 antibodies for immunohistochemical studies (Abcam, USA). Results. Our results show that with ulcerative colitis in 37.5 % of cases IgG4-positive plasma cells in the colon mucosa have not been identified. In 25 % of cases, sporadic IgG4-positive plasma cells were identified. In 37.5 % of cases, the groups of IgG4-positive plasma cells not exceeding 5 cells in one group were found. In Crohn’s disease, groups of IgG4-positive plasma cells were observed in all cases, in addition it should be noted that the group included 10 or more cells. Conclusions. It is shown that in UC, IgG4-positive plasma cells may be absent, solitary or gathered in small groups to 5 cells, and in CD, the groups consisting of 10 or more cells are observed.


IgG4-positive plasma cells; ulcerative colitis; Crohn’s disease

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Adler G. Bolezn Krona i yazvennyiy kolit [Crohn's disease and ulcerative colitis]. Мoscow: GEOTAR Media; 2001. 500p. (in Russian)

Belousova EA. Yazvennyiy kolit i bolezn Krona [Ulcerative colitis and Crohn's disease]. Tver: Triada; 2002. 128 p. (in Russian)

Halif IL, Loranska ID. Vospalitelnyie zabolevaniya kishechnika (nespetsificheskiy yazvennyiy kolit i bolezn Krona). Klinika, diagnostika i lechenie. [Inflammatory bowel disease (ulcerative colitis and Crohn's disease). The clinic, diagnosis and treatment]. Мoscow: Miklosh; 2004. 88 p. (in Russian)

Stepanov YuM, Stoykevich MV, Sorochan OV. Application of 5-Aminosalicylic Acid Preparations in the Treatment of Inflammatory Bowel Diseases. Hastroenterolohiya. 2016;3(61):80-88. doi: 10.22141/2308-2097.3.61.2016.79163. (in Ukrainian)

Stepanov YuM, Gaydar YuA. Immunoglobulin G4-associated sclerosing disease of the digestive system. Vesnik akademii meditsinskih nauk Ukrainyi. 2015;21(1):54-60. (in Russian)

Silverberg MS, Satsangi J, Ahmad T, et al. Toward an integrated clinical, molecular and serological classification of inflammatory bowel disease: report of a working party of the 2005 Montreal World Congress of Gastroenterology. Can J Gastroenterol. 2005 Sep;19 Suppl A:5A-36A. PMID: 16151544.

Schukina OB. Indeterminate colitis: current status of the problem. Consilium Medicum Ukraina. 2009;3(7):3-5. (in Russian)

Stoykevich MV. Effect of Nutritional Status Disorders on the Quality of Life in Patients with Chronic Inflammatory Bowel Diseases. Hastroenterolohiya. 2016;1(59):77-81. doi: 10.22141/2308-2097.1.59.2016.74532. (in Ukrainian)

Deshpande V. The pathology of IgG4-related disease:critical issues and challenges. Semin Diagn Pathol. 2012 Nov;29(4):191-6. doi: 10.1053/j.semdp.2012.08.001.

Sato Y, Notohara K, Kojima M, Takata K, Masaki Y, Yoshino T. IgG4-related disease: historical overview and pathology of hematological disorders. Pathol Int. 2010 Apr;60(4):247-58. doi: 10.1111/j.1440-1827.2010.02524.x.

Virk R, Shinagare S, Lauwers GY, Yajnik V, Stone JH, Deshpande V. Tissue IgG4-positive plasma cells in inflammatory bowel disease: a study of 88 treatment-naive biopsies of inflammatory bowel disease. Mod Pathol. 2014 Mar;27(3):454-9. doi: 10.1038/modpathol.2013.121.

Ravi K, Chari ST, Vege SS, et al. Inflammatory bowel disease in the setting of autoimmune pancreatitis. Inflamm Bowel Dis. 2009 Sep;15(9):1326-30. doi: 10.1002/ibd.20898.

Gaidar YA, Oshmyanska NY, Halenko A.P. Histostructure of pancreas in patients with autoimmune pancreatitis type I and II: connection with the level of IgG4-positive plasma cells. Patologiya. 2014;30:68-71. (in Russian)

Bueverov AO, Kucheriavyi YA. IgG4-assotsiirovannaia bolezn': monografiia [IgG4-associated disease: a monograph]. Moskow: Forte Print; 2014. 128 p.

Kuwata G, Kamisawa T, Koizumi K, et al. Ulcerative colitis and immunoglobulin G4. Gut Liver. 2014 Jan;8(1):29-34. doi: 10.5009/gnl.2014.8.1.29.

Guarneri F, Guarneri C, Benvenga S. Helicobacter pylori and autoimmune pancreatitis: role of carbonic anhydrase via molecular mimicry? J Cell Mol Med. 2005 Jul-Sep;9(3):741-4. PMID: 16202223.

Akitake R, Watanabe T, Zaima C. Possible involvement of T helper type 2 responses to Toll-like receptor ligands in IgG4-related sclerosing disease. Gut. 2010 Apr;59(4):542-5. doi: 10.1136/gut.2009.200972.

Deshpande V, Chicano S, Finkelberg D, et al. Autoimmune pancreatitis: a systemic immune complex mediated disease. Am J Surg Pathol. 2006 Dec;30(12):1537-45. doi: 10.1097/01.pas.0000213331.09864.2c.

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